Abstract

During early telencephalic development, intricate processes of regional patterning and neural stem cell (NSC) fate specification take place. However, our understanding of these processes in primates, including both conserved and species-specific features, remains limited. Here, we profiled 761,529 single-cell transcriptomes from multiple regions of the prenatal macaque telencephalon. We deciphered the molecular programs of the early organizing centers and their cross-talk with NSCs, revealing primatebiased galanin-like peptide (GALP) signaling in the antero-ventral telencephalon. Regional transcriptomic variations were observed along the fronto-temporal axis during early stages of neocortical NSC progression and in neurons and astrocytes. Additionally, we found that genes associated with neuropsychiatric disorders and brain cancer risk might play critical roles in the early telencephalic organizers and during NSC progression.

Citation

If you use the data in your research, please cite:

Micali, N.^†, Ma, S.^†, Li, M.^†, Kim, S.-K., Mato-Blanco, X., Sindhu, S.K., Arellano, J.I., Gao, T., Shibata, M., Gobeske, K.T., Duque, A., Santpere G., Sestan, N.^*, Rakic, P^*. (2023). Molecular programs of regional specification and neural stem cell fate progression in macaque telencephalon.

Data Availability

The scRNA-seq data were deposited in the NeMO Archive under identifier nemo:dat-fjx1jbr , as well as NCBI GEO: GSE226451

Code Availability

The scripts are deposited at Github: https://github.com/sestanlab/Macaque_corticogenesis_scRNA-seq

Contact

For any issues, please contact Shaojie Ma (j.ma@yale.edu); Sestan lab and Rakic lab at Yale University